The determination of the cutoff frequency in real solar observations under different local physical conditions is an important and underexplored aspect of waves in solar physics. This work utilizes the near ultraviolet (NUV) spectrum of the quiet sun (QS), observed by the Interface Region Imaging Spectrograph (IRIS) on November 16th, 2013, in sit-and-stare mode. It contains several absorption and emission lines that form at different heights between the photosphere and chromosphere. Cross-wavelet analysis is performed on Doppler velocity time series of pairs of spectral lines sampling different atmospheric layers to estimate the cutoff frequency at six different heights between the photosphere and chromosphere. It is found that the cutoff frequency increases with height from around 3.0 mHz at 0.38 Mm (photosphere) to around 8.5 mHz at 1.2 Mm (chromosphere). Higher chromospheric heights show indications of standing oscillations. The presented observational results are compared with those previously obtained, and serve as a benchmark to refine theoretical models that predict variations of cutoff frequencies in the solar atmosphere.