High-precision multi-band Hubble Space Telescope (HST) photometry reveals that the main sequence of the globular cluster NGC 6397 splits into two components, containing ~30% and ~70% of the stars. This double sequence is consistent with the idea that the cluster hosts two stellar populations: (1) a primordial population that has a composition similar to field stars, containing ~30% of the stars, and (2) a second generation with enhanced sodium and nitrogen, depleted carbon and oxygen, and a slightly enhanced helium abundance (ΔY ~ 0.01). We examine the color difference between the two sequences across a variety of color baselines and find that the second sequence is anomalously faint in m F336W. Theoretical isochrones indicate that this could be due to NH depletion. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA, Inc., under NASA contract NAS 5-26555.